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鏈霉親和素的介紹
2019-06-08 08:21
鏈霉親和素(streptavidin下稱SA)是與親和素(avidin下稱AV)有相似生物學(xué)特性的一種蛋白質(zhì)，是streptomyces avidinii菌的分泌物，其分子量及結(jié)合生物素的能力與雞蛋清中的親和素相似，等電點(diǎn)6.0，非特異性結(jié)合遠(yuǎn)比親和素低。
SA 是Streptomyces avicllrdi菌培養(yǎng)過程中的分泌產(chǎn)物，主要通過2一亞氨基生物素親和層析法提純，1L培養(yǎng)液中含蛋白
鏈霉親和素是四聚體蛋白，大小為66KDa。一分子鏈霉親和素可以高度特異性地與四分子生物素結(jié)合，兩者之間的親和力極為強(qiáng)烈，鏈霉親和素-生物素復(fù)合物的解離常數(shù)處于 10-14 mol/L 數(shù)量級(jí) [1] ，這一性質(zhì)常用于分子生物學(xué)用途。其中一條完整的SA肽鏈中有159個(gè)氨基酸殘基，分子量為16450。
Streptavidin(SA) is a protein that has similar biological properties to avidin(AV). It is a secretion of streptomyces avidini, and its molecular weight and ability to bind biotin are similar to those in egg whites. At 6.0 isoelectric points, the non-specific binding is much lower than the affinity.
SA is a secretory product during the culture of Streptomyces avicllrdi bacteria. It is mainly purified by the 2-imino biotin affinity chromatography, and the 1L culture solution contains proteins.
Streptomycin is a tetrameric protein with a size of 66 KDa. One molecule of streptomycin can bind to four molecules of biotin with high specificity, and the affinity between the two is extremely strong. The dissociation constant of the streptomycin-biotin complex is in the order of 10-14 MOL / L[ 1] This property is often used for molecular biology purposes. One of the complete SApeptide chains has 159 amino acid residues with a molecular weight of 16450.
Streptavidin(SA) is a protein that has similar biological properties to avidin(AV). It is a secretion of streptomyces avidini, and its molecular weight and ability to bind biotin are similar to those in egg whites. At 6.0 isoelectric points, the non-specific binding is much lower than the affinity.
SA is a secretory product during the culture of Streptomyces avicllrdi bacteria. It is mainly purified by the 2-imino biotin affinity chromatography, and the 1L culture solution contains proteins.
Streptomycin is a tetrameric protein with a size of 66 KDa. One molecule of streptomycin can bind to four molecules of biotin with high specificity, and the affinity between the two is extremely strong. The dissociation constant of the streptomycin-biotin complex is in the order of 10-14 MOL / L[ 1] This property is often used for molecular biology purposes. One of the complete SApeptide chains has 159 amino acid residues with a molecular weight of 16450.
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